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aFGF

Fibroblast Growth Factor Acidic
Recombinant, Human

 

Description

 

Recombinant human fibroblast growth factor –acidic (FGF-1, aFGF ) is a single polypeptide chain containing 141 amino acids and having a molecular mass of 15.5kDa. The sequence of this recombinant protein is identical to natural aFGF, except it contains a methionine residue attached to the N-terminus.

INCI Name

:

rh-polypeptide-11

Synonyms

:

aFGF, FGF-1, retina-derived growth factor,

heparin-binding growth factor (class I or alpha),

astroglial growth factor I

Trade Name

:

MC-aFGF

Functions and Uses

 

1. Anti-wrinkle and Anti-aging

(a) aEGF modulates the skin rejuvenating by kstimulation of cell proliferation

(b) aEGF rejuvenates both the epidermal cells and the underlying fibroblast cells, which produce collagen, elastin, and hyaluronic acid, maintain human skin elasticity, and decrease cutaneous senescence.

(c) The proliferation of epidermal cells enhanced the skin cells regeneration.

2. Wound healing and tissue repair

Angiogenic in vivo, stimulate cell proliferation on human vein endothelial cell, induce the formation of new capillary blood vessels. It binds to heparin, which potentiates its biological activity and protects it from proteolysis.

3. Rejuvenates hair follicle to produce new hair

aFGF can activate FGF-7, promote hair growth by stimulation of cell proliferation on primary hair cell, treat or prevent baldness.

4. Mitogenic for a variety of mesenchymal and neuroectodermal cells

It promotes DNA synthesis, stimulate cell proliferation and differentiation associated with wound healing, angiogenesis, tissue regeneration, embryogenesis and CNS development.

Interactions

Alcohol and iodine may probably destroy the characteristic properties of aFGF which lead to the decrease of its activity. The skin on which Alcohol or iodine are used should be washed with normal saline prior to apply aFGF.

Specification

 

Appearance

:

White lyophilized powder mass

 

Molecular weight

:

15.5k Da

 

Purity ( HPLC and SDS-PAGE)

:

95% min

 

Activity

:

Between 90% and 200% of 250000 IU/mg

 

pH

:

5.8-6.2

 

Preservative

:

Absent

 

Sterility Test

:

Sterile

 

Package

:

0.1mg;1.0mg and 10mg in ampoule or tiny bottle pack

 

Storage

:

Store the lyophilized product at -20°C, repeated freezing and thawing is not recommended.

 

Shelf life

:

36 months on its original package at 2-8°C

 

Reconstitution

:

Reconstitute the product with a specially prepared solution, store at 2-8°C and prevent from microbial contamination.

EGF Formulation Suggestions

Trace dilution method

 

Since the amount of aFGF added to cosmetics is very small, it is not suitable to directly add frozen dry mass to cosmetics. Trace dilution method is suggested which dissolve aFGF frozen dry mass having a known purity in a solvent at a temperature of 5-10°C and dilute with the same solution to have a concentration of 10ppm. The diluted solution may be stored in freezer of under low temperature. The diluted solution should be protected from being contaminated by bacteria since aFGF is easily hydrolyzed and losses its activity by enzyme produced by bacteria.

Temperature

 

MC-aFGF is relatively stable to temperature by adding special stabilizer in it. Still, we recommend that MC-aFGF is added to the formulation at a relatively low temperature (below 40°C).

pH

 

aFGF is sensitive to pH of cream. Prior to adding diluted aFGF solution to cream, the pH value of cream should be adjusted to 5-8. since at pH below 5 and above 8 and temperature 25℃, aFGF easily forms polymerid and losses activity.

Microbial Attack

 

aFGF is easily decomposed by enzymes produced by microorganisms and loses its activity. So it is extremely important to avoid microbial contamination during processing of products containing aFGF as well as storage and transportation.

Compatibility

The following ingredients may stabilize aFGF or potentiate its activity in formulation so may be considered in designing formulation.

 

Ingredients

% wt.

Function

 

Heparin

 

Potentiate aFGF activity

 

Dithiothreitol (DTT)

10mM

Anti-oxidation

 

Hyaluronic  acid

5

Humectants

 

Mannitol

5

Stabilize aFGF

 

dextran

4

Stabilize aFGF

 

Hydorolyzed collagen

1-2

Potentiate aFGF activity

 

N-acetyl cysteine

0.5

Protect aFGF from hydrolysis
by enzyme on the skin

 

Glycine

2

 

 

Azone

q.s.

Penetration enhancer

 

Propylene  glycol

q.s.

Penetration enhancer

 

Sucrose

1

Stabilizer

 

Sodium citrate

10mM

Stabilizer

 

EDTA

1mM

Stabilizer

 

Tris

q.s.

Buffer, maintain pH 

 

phosphate-buffered saline (PBS) containing 0.1% BSA or HAS

q.s.

Buffer, maintain pH

Use Level:  

 

Repairing products

:

10-15μg/ml

 

Skin care / Serum / Cream

:

1-5μg/ml

 



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